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In T1D, autoreactive T cells develop in the pancreatic lymph nodes, where dendritic cells and, eventually, B cells, serve as antigen-presenting cells (APCs) to CD4+ T cells via MHC class II proteins ( Although autoreactive T cells are a hallmark of T1D, preproinsulin-specific CD8+ T cells are present at comparable levels in the exocrine pancreas of healthy, AAb+, and T1D patients ( high autoreactive memory T cells have also been reported in the pancreatic lymph nodes of NOD mice