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For colony formation assay, cells were cultured in 24-well tissue culture plates for a week to form colonies before being fixed with 10% neutral formalin, stained with 0.5% crystal violet solution, and having the dye extracted by adding 10% acetic acid

[DOI] [PMC free article] [PubMed] [Google Scholar] 31.Pritzker K.P., Gay S., Jimenez S.A., et al

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An injection bypasses this process, ensuring you get the entire dose effectively every time