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The lysates were centrifuged (10.000 g , 30 min, 4 C) and the supernatants were loaded on equilibrated Ni-NTA agarose columns (Qiagen)

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The mechanism involves B6 role in neurotransmitter synthesis, particularly serotonin production, which influences the signaling pathways between the gut and the brain that trigger the nausea response

Diabetes Nutr Metab 17:336342 Yasuda N, Inoue T, Nagakura T et al (2002) Enhanced secretion of glucagon-like peptide 1 by biguanide compounds