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WJ-39, an Aldose Reductase Inhibitor, Ameliorates Renal Lesions in Diabetic Nephropathy by Activating Nrf2 Signaling

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To determine the effect of the V229A substitution on the overall GLP-1R binding to cholesterol in active versus inactive conditions, cells from both sublines were labelled with PhotoClick cholesterol and exposed to UV light prior to SNAP/FLAG-hGLP-1R purification by anti-FLAG immunoprecipitation and click chemistry to fluorescently label covalently bound PhotoClick cholesterol (Figure 3C)