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Statistical analysis All data were presented as mean standard deviation (SD) from at least three independent experiments unless otherwise stated, and concentration response curves were plotted using GraphPad Prism software

With respect to age-related diseases, such as AD, and advancing age, it was indicated that the melatonin concentrations in individuals reduce, which is highly correlated with disease progression and genotype (Reiter 1992

PubMed Central PMCID: PMCPMC4066722

doi: 10.7196/SAMJ.2020.V111I11.15433 24 KostkaKRoelETrinhNTHMercad-BesoraNDelmestriAMateuLet al

The organic layer was reconstituted in chloroform:methanol (2:1 v/v, 200 l and diluted 1:10 with 2:1:1 isopropanol:acetonitrile:water, with the addition of an internal standard mix composed of isotopically labelled compounds from major lipid classes