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A total of 800 puffs (100 puffs per filter pad) were puffed (100-ml puff volume, 4-s puff duration and 40-s interval between puffs) and final EC extracts were prepared as described above

On the contrary, mtDNA was released by cell debris and EVs (Figure 3B, E) , trended with decreased mitochondrial membrane, increased superoxide, and protein oxidation (Figure 4)

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