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The ER was implicated as a site for virus replication complex (VRC) formation through co-localization of an ER marker, BiP, with MP-GFP and in turn, MP-GFP co-localization with replication proteins during immunofluorescence studies (Reichel and Beachy (1998), using transgenic plants expressing an ER-targeted GFP, determined that the ER formed large cortical aggregates at reticulate vertices and fewer membrane tubules during TMV accumulation, but returned to a normal structure after replication ended

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