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Cells were trypsinized, pelleted, and washed with ice-cold phosphate-buffered saline (PBS) followed by resuspension in radioimmunoprecipitation assay (RIPA) buffer supplemented with 1X Protein Inhibitor Cocktail and phenylmethylsulfonyl fluoride (PMSF) (Roche)

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Concurrently, silca wicks slowly gave way to cotton wicks, as silca contained impurities that were determined to be harmful when inhaled