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A paradigm shift occurred in 2013 when Shokat and colleagues identified a cryptic pocket adjacent to the Switch II region in the KRAS G12C mutant, enabling the development of covalent inhibitors that irreversibly engage the mutant cysteine
[44] A third study, which was completed in 2014 found reduced decline in lung function and IPF disease progression
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Biochemical and Biophysical Methods in Molecular and Cellular Biology
Next, 40 mL of seed culture grown overnight was added to 4 L of LB broth and incubated at 37 C until it reached an optical density of 0.6 as measured at 600 nm ( A 600 )
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