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The randomized, double-blind, placebo-controlled clinical trial is the keystone of drug development to determine clinical efficacy, and increasing interest is being paid to effectiveness studies that take into account real-world factors (including patient adherence)

Tovo-Rodrigues L, Schneider BC, Martins-Silva T, Del-Ponte B, Loret de Mola C, Schuler-Faccini L, et al

PMID: 21305538

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After the NAC/FA treatment, the solutions were centrifuged at 200 g for 1 min, followed by two washing steps with 5 mL M9 medium