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Finally, the interaction of TXNDC12 with GGT7 was predicted by STRING database and confirmed by CO-IP assay, the effect of TXNDC12 on ferroptosis through GGT7 was evaluated by GSH assay, MDA assay, ROS assay and Western blot

56%) ( P =0.0001)

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2.10 Immunofluorescence assay Cells were fixed with 4% paraformaldehyde for 15 minutes, permeabilized with 0.5% Triton X-100 for 20 minutes, and blocked with 10% goat serum for 30 minutes

Methionine helps synthesize glutathione, a natural antioxidant produced in the liver that helps eliminate toxins and speeds up fat removal within the liver, promoting liver function