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The membrane was blocked with 5% skim milk for 1 h at room temperature and incubated with primary antibodies Bax, Bcl-2, Keap1, Nrf2, ZO-1, E-cadherin (1:1000 dilution) overnight at 4 , followed by HRP-conjugated secondary antibodies for additional 1 h at 37

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to project the life expectancy of HIV patients based on smoking status, sex and age at entry to HIV care in cohorts of 1 million individuals