Control of BACE1 degradation and APP processing by ubiquitin carboxyl-terminal hydrolase L1
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The thermal cycling comprised an initial denaturation step at 95 C for 4 min, followed by 35 cycles at 95 C for 30 s, 59 C for 30 s and 72 C for 30 s, with the final extension at 72 C for 7 min
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