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10.1016/j.lfs.2022.120861 20 AtarashiK.TanoueT.ShimaT.ImaokaA.KuwaharaT.MomoseY.et al

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When the cells were expanded to ~7590% confluence, the cells were washed twice with PBS to remove the residual serum, digested and centrifuged and resuspended with sterile PBS, the cells were counted and the cell concentration was adjusted to 110 7 /ml, and the cell suspension was stored in EP tubes and kept on ice

Notably, supplementation with ferrostatin-1 or trolox, both of which are radical-trapping antioxidants and potent inhibitors of ferroptosis, rescued survival but not proliferation under cystine-free conditions (Extended Data Fig

Always use your product's actual labeled strength, its specific reconstitution instructions, and the exact dose your prescriber has given you not figures from an example