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A baseline reading was taken for 1 minute, then 100 nM exendin-4 was added and cells/islets imaged for a further 5 minutes, prior to addition of 100 M isobutyl methylxanthine (IBMX) + 10 M forskolin (FSK) and 2-minute imaging for maximal response

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GLP-1 is released by the gut after eating, and it helps to regulate blood sugar levels and appetite