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The protein contents of the samples were determined by Bio-Rad Protein Assay (Bio-Rad Laboratories, Richmond, CA) and ALP activity was expressed as arbitrary units relative to protein content (units/mg)

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Abstract This introductory chapter presents some basic information about tobacco and nicotine products, procedures for smoke collection (FTC, ISO, HCI), a general discussion about the chemical composition of tobacco, tobacco smoke, e-liquids and aerosols from ENDS, discusses the list of harmful and potential harmful compounds (HPHCs) as indicated by the US FDA, and presents several reference cigarettes and tobacco products as generated by Kentucky Tobacco Research & Development Center of the University of Kentucky