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Following phase separation, the supernatant organic solution was used to make nicotine working standards in concentrations of 50, 250, 500, 750, 1000 and 1500 mg/L, which were serially diluted with the extraction solution
Importantly, the process does not involve the CYP450 enzyme system, showing no inhibitory or inductive effect on clinically significant isoforms such as CYP1A2, CYP2C9, CYP2C19, CYP2D6, CYP2E1, or CYP3A4
Ratsch AM, Mason A, Rive L, Bogossian FE, Steadman KJ
Connolly GN, Richter P, Aleguas A Jr, Pechacek TF, Stanfill SB, Alpert HR
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