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Trichoplusia ni cell pellets were suspended in 30 mM HEPES (pH 7.4), 50 mM NaCl, 5 mM CaCl 2 , 2 mM MgCl 2 supplemented with protease inhibitors (REF118361 45001, Roche) and benzonase (Merk Millipore)

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Thus, red cell 2,3-BPG levels are exquisitely sensitive to pH: a rise in pH causes a rise in 2,3-BPG levels, whereas acidosis results in 2,3-BPG depletion