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10 ul of each drug solution was added to 90 ul of the rat blood serum samples and at specific time intervals the researchers terminated metabolism by adding 1 ml of ACN and acetic acid in order to precipitate the proteins which were then removed after overnight refrigeration followed by centrifugation

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Pathological investigation of sarcoid SFN has documented a loss of small myelinated (A) and unmyelinated (C) fibers of the sensory and autonomic nervous systems (2), as well as both sensory and motor fibers (3)