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The demultiplexed NGS reads were trimmed with cutadapt (v4.6) (Martin, 2011) to retrieve the cDNA barcode or the sgRNA sequences, and the reads were mapped and counted using MAGeCK count (v0.5.9.2.4) (Li et al, 2014) and normalized on total read counts

Colesevelam in DIO mice, TGR5-mediated induces GLP-1 secretion and improved glucose tolerance [184, 185]

The injection itself takes under one minute