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The approach used for HbA1c progression and treatment intensification in the present study was chosen to accurately reflect real-world clinical practice where treatments are continued while glycemic control is maintained, and intensified as type 2 diabetes naturally progresses over patients lifetimes [14, 15, 30]

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After more vigorous pipetting, cells were pelleted by centrifugation at 400 RCF for 5 min and seeded onto a 24-well plate