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The shRNA target sequences were 5-GGAUUGCUCUAGCCUGUAATT-3 (#1) and 5-GCUGCUGCCUAUUGUAGAUTT-3 (#2)

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The finding established that the GSH redox cycle is the primary intracellular antioxidant defence system in endothelial cells against peroxide-mediated oxidative stress, and remains a foundational reference for endothelial oxidative stress research using GSH manipulation

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