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Targeted LC-MS/MS is suitable for focused hypotheses requiring rigorous stabilization and quantification, whereas discovery-oriented studies may combine broader profiling with targeted follow-up

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Fas-associated via death domain and TNFRSF1A associated via death domain (TRADD) facilitate the recruitment of initiator caspase 8 (CASP8) or caspase 10 (CASP10), resulting in the creation of a death-inducing signaling complex and subsequent activation of procaspase