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Preparation of the muscle specimens and frozen section To prepare frozen muscle sections, we first anesthetized adult fish with a 0.025% buffered MS-222 solution (Sigma-Aldrich, US), and then removed a segment of muscle tissue located between the anal and caudal fins 35

Experts, including Kelsey Costa, MS, RDN, registered dietitian nutritionist and founder of Dietitian Insights, have noted that eggs are a great source of protein and various micronutrients

This method is particularly effective for individuals with absorption issues, such as those with gastrointestinal disorders or older adults with decreased gastric acid production

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Activity of single neurons in the hypothalamic feeding centers: effect of glucose