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2001;131(3s):955S-962S

Representative tissue specimens were collected, dehydrated and embedded in parafn blocks, sectioned at 4 m, and stained with hematoxylin-eosin according to the following automated Sakura Tissue-Tek DRS 2000 Slide Stainer protocol ( Coverslipping/H-E-Kit accessed on 19 October 2021): rehydration in distilled water, stain- Biomedicines 2022,10, 265 6 of 38 ing with hematoxylin, washing in running tap water, differentiation with 70% alcohol, staining with eosin, dehydration, clearing, and mounting

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Samples were then heated for 15 min at 70 C

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