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All analytes were separated on an ACQUITYUPLC BEH Amide column (2.1 mm 100 mm, 130 , 1.7 m, Waters Corporation, Milford, Massachusetts) with an upstream Van Guard UPLC BEH Amide pre-column (2.1 mm 5 mm, 1.7 m, Waters Co., Milford, MA) using water and acetonitrile/water as mobile phases

However, many current studies are primarily focused on improving the pharmacodynamic and pharmacokinetic properties of copper, while data on copper-induced dermal toxicity remain limited

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This finding, together with recent guideline updates, may explain the improved glycemic control observed in the later study period