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Absolute quantification of ubiquitin chain linkages Followed the standardized protocol for parallel reaction monitoring (PRM)-based measurement of endogenous ubiquitin chain linkages using Absolute QUAntification (AQUA) synthetic peptides, as described by us before 5 , with minor modifications

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Subsequent mass spectrometry analysis of biotin-captured proteins revealed that FBXO22 was the strongest interactor

Multiple case reports or clinical trials have documented the use of metreleptin as a treatment (3, 37, 38, 39, 40)

LncRNA VINRs capacity to bind to cactin, preventing its degradation by the ubiquitin-proteasome and promoting noncanonical antiviral and AMP defense, reduced viral multiplication (228)